Open AccessUltrasound Triggered Drug Release from Affinity-Based β-Cyclodextrin Polymers for Infection Control
Author(s) -
Smriti Bohara,
Nathan A. Rohner,
Emily Budziszewski,
Jackrit Suthakorn,
Horst A. von Recum,
Agata A. Exner
Publication year - 2021
Publication title -
annals of biomedical engineering
Language(s) - English
Resource type - Journals
eISSN - 1573-9686
pISSN - 0090-6964
DOI - 10.1007/s10439-021-02814-y
Subject(s) - drug delivery , staphylococcus aureus , antibiotics , drug , cyclodextrin , in vitro , chemistry , rifampicin , ultrasound , pharmacology , biomedical engineering , microbiology and biotechnology , bacteria , medicine , chromatography , biology , biochemistry , genetics , organic chemistry , radiology
This work demonstrates a slow, sustained drug delivery system that provides on-demand delivery bursts through the application of pulsed therapeutic ultrasound (TUS). Insoluble β-cyclodextrin-polymer (pCD) disks were loaded with a saturated antibiotic solution of rifampicin (RIF) and used for drug delivery studies. To obtain on-demand release from the implants, TUS was applied at an intensity of 1.8 W/cm 2 . The therapeutic efficacy of the combination treatment was assessed in bacterial culture via an in vitro Staphylococcus aureus bioluminescence assay. The results demonstrated that the application of pulsed TUS at 3 MHz and 1.8 W/cm 2 to pCD implants leads to a significantly higher short-term burst in the drug release rate compared to samples not treated with TUS. The addition of TUS increased the drug release by 100% within 4 days. The pCD disk + RIF stimulated with TUS showed a comparatively higher bacterial eradication with CFU/mL of 4.277E+09, and 8.00E+08 at 1 and 24 h compared with control treated bacteria at 1.48E+10. Overall, these results suggest that the addition of pulsed TUS could be an effective technology to noninvasively expedite antibiotic release on demand at desired intervals.