Isolation and structure determination of a new lantibiotic cinnamycin B from Actinomadura atramentaria based on genome mining | Zendy

Shinya Kodani | Zendy; Hisayuki Komaki | Zendy; Sho Ishimura | Zendy; Hikaru Hemmi | Zendy; Mayumi OhnishiKameyama | Zendy

Open Access

Isolation and structure determination of a new lantibiotic cinnamycin B from Actinomadura atramentaria based on genome mining

Author(s) -

Shinya Kodani,

Hisayuki Komaki,

Sho Ishimura,

Hikaru Hemmi,

Mayumi OhnishiKameyama

Publication year - 2016

Publication title -

journal of industrial microbiology and biotechnology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.857

H-Index - 112

eISSN - 1476-5535

pISSN - 1367-5435

DOI - 10.1007/s10295-016-1788-9

Subject(s) - lantibiotics , genome , lanthionine , chemistry , streptomyces , isolation (microbiology) , gene cluster , stereochemistry , bacteria , biology , gene , microbiology and biotechnology , biochemistry , genetics , bacteriocin

New lantibiotic cinnamycin B was isolated from the extract of Actinomadura atramentaria NBRC 14695(T), based on genome mining and chemical investigation. The partial structure of cinnamycin B was established by 2D NMR experiments, which indicated that cinnamycin B had same methyl lanthionine bridging pattern with cinnamycin. The reduction with NaBH4-NiCl2 afforded the reduced cinnamycin B, and MS/MS experiment indicated the presence of hydroxy asparatic acid in the molecule. Cinnamycin B showed an antibacterial activity against Streptomyces antibioticus with dosage of 5 μg (0.5μL, 10 mg/mL solution) at spot-on-lawn testing method. The gene cluster of cinnamycin B on the genome of A. atramentaria was identified and discussed in comparison with that of cinnamycin.

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