Purification and biological characterization of halocin H1 from Haloferax mediterranei M2a | Zendy

Gonzalo Platas | Zendy; Inmaculada Meseguer | Zendy; Ricardo Amils | Zendy

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Purification and biological characterization of halocin H1 from Haloferax mediterranei M2a

Author(s) -

Gonzalo Platas,

Inmaculada Meseguer,

Ricardo Amils

Publication year - 2002

Publication title -

international microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.578

H-Index - 65

eISSN - 1618-1905

pISSN - 1139-6709

DOI - 10.1007/s10123-002-0053-4

Subject(s) - bacitracin , chemistry , bacteriocin , archaea , microbiology and biotechnology , halobacteriaceae , biochemistry , hydroxylapatite , biology , antimicrobial , antibiotics , enzyme , halobacterium salinarum , gene

The production of halocins, bacteriocin-like proteins of ecological significance, is a frequent characteristic of species from the family Halobacteriaceae. Halocin H1, produced by Haloferax mediterranei strain M2a, is a single 31-kDa polypeptide. Its purification was achieved by combining two chromatographic systems: Sepharose 4B linked to bacitracin followed by hydroxylapatite Bio-gel HTP. Halocin H1 required concentrations of NaCl higher than 1.5 M to maintain its activity. Haoarchaeal strains showed a differential degree of sensitivity to the action of this halocin.

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