DHA-enriched phosphatidylcholine suppressed angiogenesis by activating PPARγ and modulating the VEGFR2/Ras/ERK pathway in human umbilical vein endothelial cells | Zendy

Yuanyuan Liu | Zendy; Yingying Tian | Zendy; Yao Guo | Zendy; Ziyi Yan | Zendy; Changhu Xue | Zendy; Jingfeng Wang | Zendy

Open Access

DHA-enriched phosphatidylcholine suppressed angiogenesis by activating PPARγ and modulating the VEGFR2/Ras/ERK pathway in human umbilical vein endothelial cells

Author(s) -

Yuanyuan Liu,

Yingying Tian,

Yao Guo,

Ziyi Yan,

Changhu Xue,

Jingfeng Wang

Publication year - 2021

Publication title -

food science and biotechnology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.595

H-Index - 38

eISSN - 2092-6456

pISSN - 1226-7708

DOI - 10.1007/s10068-021-00990-0

Subject(s) - angiogenesis , umbilical vein , mapk/erk pathway , chemistry , chorioallantoic membrane , neovascularization , cd36 , microbiology and biotechnology , peroxisome proliferator activated receptor , biochemistry , receptor , signal transduction , biology , cancer research , in vitro

Docosahexaenoic acid-enriched phosphatidylcholine (DHA-PC) is a new generation of omega-3 lipids, which contains an ester bond linking DHA at the sn-2 position of phospholipid. DHA-PC has become the interest recently as its better bioavailability and anti-oxidation capacity. In this study, the anti-angiogenic effect of DHA-PC was evaluated. The capacities of proliferation, migration, tube formation of human umbilical vein endothelial cells were significantly declined after DHA-PC treatment. Furthermore, DHA-PC inhibited the neovascularization of the chick chorioallantoic membrane in vivo. Mechanism results indicated that DHA-PC enhances the expression of peroxisome proliferator-activated receptor γ (PPARγ) at transcriptional and translational level, subsequently down-regulates the VEGFR2 expression and VEGFR2-mediated downstream Ras/ERK pathway, resulting in significant reduction in proliferation and differentiation. Additionally, PPARγ-specific antagonist GW9662 partly reversed the inhibition effects of DHA-PC on tube formation and neovascularization, suggesting that DHA-PC exerts anti-angiogenesis effect through activating PPARγ. These findings indicated that DHA-PC has a great prospect of anti-tumor angiogenesis therapy.

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