Open AccessDevelopment of enhanced selective media for detection of Vibrio parahaemolyticus in oysters
Author(s) -
JaeHyun Yoon,
YoungMin Bae,
Hana Song,
Soyul Lee,
SungKwon Moon,
SeWook Oh,
Sun-Young Lee
Publication year - 2021
Publication title -
food science and biotechnology/food science and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.595
H-Index - 38
eISSN - 2092-6456
pISSN - 1226-7708
DOI - 10.1007/s10068-021-00877-0
Subject(s) - vibrio parahaemolyticus , agar , sodium citrate , vibrio , tryptic soy broth , food science , microbiology and biotechnology , biology , bacteria , medicine , genetics , pathology
This study was undertaken to develop enhanced selective media for detection of Vibrio parahaemolyticus in oysters. Primarily, tryptic soy agar (TSA) was supplemented with 4.5-5% NaCl, 0.1-0.5% oxgall, and/or 1-2% sodium citrate, and adjusted to pH 8-9. A total of 21 Vibrio spp., 24 indicators, and 26 food-borne isolates were streaked on the modified media, followed by 24 h of incubation at 37 °C. While all the indicators and isolates failed to grow on TSA containing 5% NaCl, 0.5% oxgall, and 2% sodium citrate (TSA OSS1 ; pH 9), V. parahaemolyticus was culturable on this selective medium. Particularly, the ability of TSA OSS1 to quantify V . parahaemolyticus in oysters was superior to thiosulphate citrate bile salts sucrose (TCBS) agar. V. parahaemolyticus distinctly produced its white-yellowish, round, and edge-pointed colony on TSA OSS1 . TSAO SS1 with high selectivity potentials over TCBS may be a promising alternative for detection of V . parahaemolyticus in seafoods or natural reservoirs.