Open AccessAntimicrobial and anti-biofilm effects of probiotic Lactobacillus plantarum KU200656 isolated from kimchi
Author(s) -
Jieun Lee,
NaKyoung Lee,
HyunDong Paik
Publication year - 2020
Publication title -
food science and biotechnology/food science and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.595
H-Index - 38
eISSN - 2092-6456
pISSN - 1226-7708
DOI - 10.1007/s10068-020-00837-0
Subject(s) - listeria monocytogenes , microbiology and biotechnology , pathogenic bacteria , lactobacillus plantarum , probiotic , antimicrobial , biofilm , bacteria , staphylococcus aureus , chemistry , escherichia coli , lactobacillus , antibiotics , biology , fermentation , food science , lactic acid , biochemistry , gene , genetics
The probiotic properties and anti-pathogenic effects of Lactobacillus plantarum KU200656 (KU200656) isolated from Korean fermented kimchi against Staphylococcus aureus , Listeria monocytogenes , Escherichia coli , and Salmonella Typhimurium were investigated. KU200656 showed high tolerance to artificial gastric acid (99.48%) and bile salts (102.40%) and this strain was safe according to antibiotic sensitivity test; it could not produce harmful enzymes, including β-glucuronidase. KU200656 exhibited high adhesion (4.45%) to intestinal cells, HT-29 cells, with high cell surface hydrophobicity (87.31% for xylene and 81.11% for toluene). Moreover, KU200656 co-aggregated with pathogenic bacteria and exhibited antibacterial activity and anti-adhesion properties against pathogens. The cell-free supernatant (CFS) of KU200656 inhibited biofilm formation by pathogenic bacteria. In addition, half of the minimum inhibitory concentration of the KU200656 CFS downregulated the expression of biofilm-related genes, as determined by quantitative real-time PCR. Therefore, KU200656 was demonstrated to possess anti-pathogenic effects and have potential for use as probiotics in the food industry.