Open AccessResistance of pathogenic biofilms on glass fiber filters formed under different conditions
Author(s) -
Hana Song,
Sun-Young Lee
Publication year - 2020
Publication title -
food science and biotechnology/food science and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.595
H-Index - 38
eISSN - 2092-6456
pISSN - 1226-7708
DOI - 10.1007/s10068-020-00773-z
Subject(s) - biofilm , microbiology and biotechnology , chemistry , staphylococcus aureus , pathogenic bacteria , escherichia coli , food science , bacteria , biology , biochemistry , genetics , gene
This study investigated the survivals of two pathogens ( Escherichia coli O157:H7 and Staphylococcus aureus ) in different adhered forms on glass fiber filters (GFFs) at 43 and 68% relative humidity (RH). Efficacies of chemical sanitizers at reducing pathogenic biofilms on GFFs were also evaluated. Inoculated GFFs were incubated at 28 °C in TSB (type I), on TSA (type II), or on TSA under 100% RH (type III) to produce biofilms. When GFFs were incubated at 43 or 68% RH for 7 days, type III biofilms were less than 2 log 10 CFU/filter reduction whereas type I and type II biofilms were 4-6 log 10 CFU/filter reduction. Additionally, type III biofilms were highly resistant to sanitizing treatment compared than other biofilms (type I and II). Therefore, the method to produce biofilms used in this study could be used to produce highly resistance pathogenic biofilms in the laboratory for related experiments.