Open AccessDevelopment of a selective media for detecting Campylobacter spp. in chicken carcasses using avibactam supplemented mCCDA
Author(s) -
Binn Kim,
KunHo Seo
Publication year - 2020
Publication title -
food science and biotechnology/food science and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.595
H-Index - 38
eISSN - 2092-6456
pISSN - 1226-7708
DOI - 10.1007/s10068-020-00759-x
Subject(s) - campylobacter , cefoperazone , agar , microbiology and biotechnology , biology , activated charcoal , food science , chemistry , bacteria , antibiotics , antibiotic resistance , genetics , organic chemistry , adsorption , imipenem
Campylobacter spp. are major causes of gastrointestinal infections worldwide, and are commonly identified using modified-charcoal-cefoperazone-deoxycholate agar (mCCDA). However, the efficacy of this screening technique is often hindered by overgrowth of competing flora, such as extended-spectrum β-lactamase (ESBL)-producing Escherichia coli . Thus, in the present study we supplemented mCCDA with a recently developed ESBL inhibitor, avibactam (A-mCCDA). We inoculated mCCDA and A-mCCDA plates with 25 strains each of Campylobacter spp. and ESBL-producing E. coli , and thereby determined that the optimum avibactam concentration required to inhibit ESBL-producing E. coli was 0.0625 mg/L. At this concentration, a significantly higher proportion of Campylobacter spp. was isolated using A-mCCDA compared to that using mCCDA ( P < 0.05). Thus, the results of the present study support the use of A-mCCDA to improve current Campylobacter screening methods.