Tracking of deliberately inoculated Leuconostoc mesenteroides and Lactobacillus brevis in kimchi | Zendy

Anshul Sharma | Zendy; Jasmine Kaur | Zendy; Sulhee Lee | Zendy; YoungSeo Park | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Tracking of deliberately inoculated Leuconostoc mesenteroides and Lactobacillus brevis in kimchi

Author(s) -

Anshul Sharma,

Jasmine Kaur,

Sulhee Lee,

YoungSeo Park

Publication year - 2019

Publication title -

food science and biotechnology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.595

H-Index - 38

eISSN - 2092-6456

pISSN - 1226-7708

DOI - 10.1007/s10068-019-00719-0

Subject(s) - leuconostoc mesenteroides , rapd , biology , housekeeping gene , leuconostoc , strain (injury) , 16s ribosomal rna , lactobacillus brevis , gene , sequence analysis , dna sequencing , genetics , inoculation , microbiology and biotechnology , lactobacillus , bacteria , lactobacillus plantarum , lactic acid , population , gene expression , demography , anatomy , sociology , genetic diversity , immunology

The objective of this study was to track intentionally inoculated Leuconostoc mesenteroides (11251) and Lactobacillus brevis (B151) strains in kimchi using random amplified polymorphic DNA (RAPD), repetitive element palindromic PCR (rep-PCR), and comparative housekeeping gene sequences analysis. The 16S rRNA gene provided species-level information for 30 colonies randomly picked from kimchi inoculated with strains 11251 and B151. Out of 30 colonies, one colony was matched to strain 11251, and two colonies were found identical to strain B151 reference strain in inoculated kimchi. Notably, among the three tools, strain 11251 was best tracked by comparative gene sequence analysis, while strain B151 tracked by all three tools. Our results suggest that the gene sequence analysis is a more reliable tool for tracking of desired strains than RAPD and rep-PCR. Based on the findings, it is recommended that gene sequence analysis could be used to avoid misuse of industrially useful strains within the growing food industry.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research