Open AccessAnti-inflammatory and in vitro bone formation effects of Garcinia mangostana L. and propolis extracts
Author(s) -
Yun Kyong Lim,
So Young Yoo,
Young Yoon Jang,
Byoung Cheon Lee,
Dong Hoon Lee,
Joong-Ki Kook
Publication year - 2019
Publication title -
food science and biotechnology/food science and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.595
H-Index - 38
eISSN - 2092-6456
pISSN - 1226-7708
DOI - 10.1007/s10068-019-00697-3
Subject(s) - garcinia mangostana , propolis , chemistry , alkaline phosphatase , in vitro , porphyromonas gingivalis , traditional medicine , pharmacology , biochemistry , enzyme , biology , periodontitis , food science , medicine
The purpose of this study was to determine the anti-inflammatory and in vitro bone formation effects of Garcinia mangostana L. (mangosteen) and propolis extracts. Immortalized human gingival fibroblasts (hTERT-hNOF) cells were treated with Porphyromonas gingivalis KCOM 2804 lipopolysaccharide followed by treatment with mangosteen and propolis extract alone or in combination. Expression levels of inflammatory cytokines were evaluated by enzyme-linked immunosorbent assay. Effect of mangosteen and/or propolis extracts on mineralization of MG-63 cells was evaluated by alkaline phosphatase activity and alizarin red S staining. Group mangosteen extract complex 1:34 (1 µg/ml mangosteen extract and 34 µg/ml propolis extract) significantly reduced expression levels of IL-6, IL-8, and PGE 2 . It had higher than other groups in vitro bone formation effect on MG63 cells. These results suggest that mangosteen and propolis extract complex could be used in the prevention and treatment of periodontal disease.