Open AccessComplete genome sequences of two insect-specific flaviviruses
Author(s) -
Jermilia Charles,
Chandra S. Tangudu,
Andrew E. Firth,
Bradley J. Blitvich
Publication year - 2017
Publication title -
archives of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.943
H-Index - 110
eISSN - 1432-8798
pISSN - 0304-8608
DOI - 10.1007/s00705-017-3552-5
Subject(s) - biology , phylogenetic tree , genome , genetics , virology , sanger sequencing , complementary dna , dna sequencing , virus , whole genome sequencing , phylogenetics , multiple sequence alignment , polymerase chain reaction , sequence alignment , computational biology , gene , peptide sequence
We determined the complete genomic sequences of two previously discovered insect-specific flaviviruses, Marisma mosquito virus (MMV) and Nanay virus (NANV), using a combination of high-throughput sequencing, reverse transcription-polymerase chain reaction, 5' and 3' rapid amplification of cDNA ends and Sanger sequencing. Complete polyprotein amino acid sequence alignments revealed that the closest known relatives of MMV and NANV are Donggang virus (89% identity, 95% similarity) and Nounané virus (53% identity, 70% similarity), respectively. Phylogenetic inference is in agreement with these findings. Potential programmed -1 ribosomal frameshifting sites were bioinformatically identified in the genomes of both viruses.