Analysis of the full-length genome sequence of papaya lethal yellowing virus (PLYV), determined by deep sequencing, confirms its classification in the genus Sobemovirus
Author(s) -
Álvaro Júlio Pereira,
Poliane AlfenasZerbini,
Renan de Souza Cascardo,
E. C. de Andrade,
F. Murilo Zerbini
Publication year - 2012
Publication title -
archives of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.943
H-Index - 110
eISSN - 1432-8798
pISSN - 0304-8608
DOI - 10.1007/s00705-012-1384-x
Subject(s) - orfs , biology , genome , phylogenetic tree , genetics , whole genome sequencing , genus , nucleic acid sequence , sequence analysis , virology , complete sequence , botany , gene , peptide sequence , open reading frame
Papaya lethal yellowing virus (PLYV) causes an economically important disease in papayas in northeastern Brazil. Based on biological and molecular properties, PLYV has been tentatively assigned to the genus Sobemovirus. We report the sequence of the full-length genome of a PLYV isolate from Brazil, determined by deep sequencing. The PLYV genome is 4,145 nt long and contains four ORFs, with an arrangement identical to that of sobemoviruses. The polyprotein and CP display significant sequence identity with the corresponding proteins of other sobemoviruses. Pairwise comparisons and phylogenetic analysis based on complete nucleotide sequences confirm the classification of PLYV in the genus Sobemovirus.
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