Open AccessImmunoliposome-based fluorometric patulin assay by using immunomagnetic nanoparticles
Author(s) -
Xinjie Song,
Danhua Wang,
MyungHee Kim
Publication year - 2019
Publication title -
mikrochimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 87
eISSN - 1436-5073
pISSN - 0026-3672
DOI - 10.1007/s00604-019-3973-9
Subject(s) - patulin , chromatography , detection limit , immunoassay , chemistry , magnetic nanoparticles , immunomagnetic separation , conjugate , bovine serum albumin , microbead (research) , sulforhodamine b , nanoparticle , materials science , nanotechnology , mycotoxin , biochemistry , antibody , mathematical analysis , food science , mathematics , immunology , biology , cytotoxicity , in vitro
A fluorometric immunoassay is described for the determination of patulin, a highly toxic fungal metabolite. A rabbit anti-patulin-bovine serum albumin (BSA) IgG conjugate was prepared and used to compose immunoliposomes and immunomagnetic nanoparticles. The immunomagnetic nanoparticles are then added to the sample to form the patulin-antibody composites which can be magnetically separated. The immunoliposomes are then added to form a sandwich. After magnetic separation of the composites and adding n-octyl-β-D-glucopyranoside, the fluorophore sulforhodamine B (SRB) is released. Its fluorescence intensity was then measured at excitation/emission wavelengths of 550/585 nm. The immunoliposome-based immunomagnetic nanoparticle assay can detect 8 μg L −1 of patulin in apple juice without the need for extraction, separation, and purification. The detection limit falls within the European regulatory limit for infants and children’s products (10 μg L −1 ). The method is rapid, enviroment-friendly, and reliable.Graphical abstract Schematic of the immunoassay.An immunoliposome-patulin-immunomagnetic nanoparticle sandwich complex is formed that can be separated from the sample by magnetic force. The fluorescence of sulforhodamine B carried inside of immunoliposome was measured to determine patulin. The method can detect 8 μg L −1 of patulin in apple juice.Electronic supplementary material The online version of this article (10.1007/s00604-019-3973-9) contains supplementary material, which is available to authorized users.