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Expression of CD44 in rat liver allografts during rejection
Author(s) -
Fujisaki Shigeru,
Miyake Hiroshi,
Amano Sadao,
Nakayama Hisashi,
Oida Takatsugu,
Takizawa Hidehiro
Publication year - 1998
Publication title -
journal of hepato‐biliary‐pancreatic surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.63
H-Index - 60
eISSN - 1868-6982
pISSN - 0944-1166
DOI - 10.1007/s005340050033
Subject(s) - lymphocyte homing receptor , lymphocyte function associated antigen 1 , lymphocyte , homing (biology) , infiltration (hvac) , liver transplantation , cd44 , cell adhesion molecule , pathology , immune system , immunohistochemistry , biology , transplantation , t lymphocyte , immunology , icam 1 , medicine , intercellular adhesion molecule 1 , cell adhesion , cell , ecology , genetics , physics , thermodynamics
As CD44 is believed to be a homing receptor involved in lymphoid trafficking and inflammatory responses, it is expected to be closely linked to transplant rejection. In this study, the expression of CD44 during liver transplant rejection was compared with the expression of lymphocyte‐function associated antigen‐1 (LFA‐1) and intercellular adhesion molecule‐1 (ICAM‐1), which play an essential role in cell interactions and the initiation of immune responses. Male Brown Norway (BN) and Lewis (LEW) rats were used as donors and recipients, respectively. Orthotopic liver transplantation (OLTX) was done using the cuff technique of Kamada and Calne. Animals were killed on days 3, 5, and 7 after OLTX, and a piece of tissue from each of the liver grafts was obtained. Immunohistochemical staining was used to investigate the expression of CD44, ICAM‐1, and LFA‐1. CD44 was strongly expressed in portal areas of the rejected liver, and LFA‐1 and ICAM‐1 were expressed mainly on sinusoids and hepatocytes. These findings indicate that CD44 is closely involved in lymphocyte infiltration, which is dominant in portal areas, and that lymphocyte infiltration during the rejection process may involve a homing mechanism.