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Catalase, superoxide dismutase, and glutathione peroxidase activities in various rat tissues after carbon tetrachloride intoxication
Author(s) -
SzymonikLesiuk Stanisława,
Czechowska Grażyna,
StryjeckaZimmer Marta,
Słomka Maria,
Mądro Agnieszka,
Celiński Krzysztof,
Wielosz Marian
Publication year - 2003
Publication title -
journal of hepato‐biliary‐pancreatic surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.63
H-Index - 60
eISSN - 1868-6982
pISSN - 0944-1166
DOI - 10.1007/s00534-002-0824-5
Subject(s) - catalase , superoxide dismutase , carbon tetrachloride , glutathione peroxidase , peroxidase , chemistry , glutathione , enzyme , biochemistry , carbon tetrachloride poisoning , organic chemistry
Background The aim of this study was to determine the possible relationship between the activity of three different antioxidant enzymes — peroxidase superoxide dismutase, catalase, and glutathione peroxidase — and carbon tetrachloride‐induced injury. Methods Male Wistar rats weighing 200–250 g were used in the experiments. Rats of the experimental groups were given carbon tetrachloride 0.5 ml/kg i.p. in olive oil (5 mmol/kg body mass) for 1 or 3 days. Control group rats were injected with olive oil only for the same period. Brain, liver, kidney, and heart supernatants were used for measurement of superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPX) activities. Results No statistically significant changes in SOD and GPX activities were observed in the liver after CCl 4 administration, but catalase activity was significantly increased after 24 h and remained at that level during the course of the study. In the brain, SOD and catalase activities decreased after 24 h of experiment, but GPX activity statistically significantly increased at all time points studied. Increased activities of SOD, catalase, and GPX were found in heart after CCl 4 intoxication. The CCl 4 injection in our experiment caused a reduction of SOD and catalase activities and increased GPX activity in the kidney. Conclusions The results suggest that change in antioxidant enzyme activities may be relevant to the ability of the liver and other investigated organs to cope with oxidative stress during CCl 4 poisoning.