Expression profiling of formalin-fixed paraffin embedded primary human uveal melanomas using DASL matrices | Zendy

Sebastian Di Cesare | Zendy; André Nantel | Zendy; JeanClaude Marshall | Zendy; Bruno F. Fernandes | Zendy; E. Antecka | Zendy; María Eugenia Orellana | Zendy; D. Abourbih | Zendy; Antonia M. Saornil | Zendy; Miguel N. Burnier | Zendy

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Expression profiling of formalin-fixed paraffin embedded primary human uveal melanomas using DASL matrices

Author(s) -

Sebastian Di Cesare,

André Nantel,

JeanClaude Marshall,

Bruno F. Fernandes,

E. Antecka,

María Eugenia Orellana,

D. Abourbih,

Antonia M. Saornil,

Miguel N. Burnier

Publication year - 2009

Publication title -

journal of cancer research and clinical oncology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.338

H-Index - 94

eISSN - 1432-1335

pISSN - 0171-5216

DOI - 10.1007/s00432-009-0692-3

Subject(s) - biology , immunohistochemistry , malignancy , metastasis , immunostaining , gene expression , pathology , melanoma , microbiology and biotechnology , gene , cancer research , cancer , medicine , genetics

Fresh biopsied ocular tumor tissues are difficult to obtain for the purpose of performing microarray experiments on extracted nucleic acids. Present technology allows for extraction of total RNA from formalin-fixed paraffin embedded (FFPE) tissue analyzed by the cDNA mediated Annealing Sectioning and Ligation (DASL) method. We aimed to correlate gene transcript differences between two uveal melanoma (UM) clinical-histopathological parameters (metastasis, cell type).

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