Open AccessChromatin regulatory genes differentially interact in networks to facilitate distinct GAL1 activity and noise profiles
Author(s) -
David F. Moreno,
Murat Açar
Publication year - 2020
Publication title -
current genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.825
H-Index - 81
eISSN - 1432-0983
pISSN - 0172-8083
DOI - 10.1007/s00294-020-01124-5
Subject(s) - chromatin , biology , genetics , bivalent chromatin , gene regulatory network , chia pet , histone deacetylase , regulation of gene expression , histone , computational biology , gene , epigenetics , trichostatin a , phenotype , gene expression , chromatin remodeling , microbiology and biotechnology
Controlling chromatin state constitutes a major regulatory step in gene expression regulation across eukaryotes. While global cellular features or processes are naturally impacted by chromatin state alterations, little is known about how chromatin regulatory genes interact in networks to dictate downstream phenotypes. Using the activity of the canonical galactose network in yeast as a model, here, we measured the impact of the disruption of key chromatin regulatory genes on downstream gene expression, genetic noise and fitness. Using Trichostatin A and nicotinamide, we characterized how drug-based modulation of global histone deacetylase activity affected these phenotypes. Performing epistasis analysis, we discovered phenotype-specific genetic interaction networks of chromatin regulators. Our work provides comprehensive insights into how the galactose network activity is affected by protein interaction networks formed by chromatin regulators.