Open AccessOpportunities for new studies of nuclear DNA replication enzymology in budding yeast
Author(s) -
Marta A. Garbacz,
Scott A. Lujan,
Thomas A. Kunkel
Publication year - 2019
Publication title -
current genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.825
H-Index - 81
eISSN - 1432-0983
pISSN - 0172-8083
DOI - 10.1007/s00294-019-01023-4
Subject(s) - biology , dna replication , dna polymerase , dna polymerase ii , genetics , polymerase , dna polymerase delta , control of chromosome duplication , dna clamp , microbiology and biotechnology , exonuclease , eukaryotic dna replication , proofreading , computational biology , dna , gene , polymerase chain reaction , reverse transcriptase
Three major eukaryotic DNA polymerases, Polymerases α, δ, and ε (Pols α, δ, and ε), perform the fundamental process of DNA synthesis at the replication fork both accurately and efficiently. In trying to understand the necessity and flexibility of the polymerase usage, we recently reported that budding yeast cells lacking Pol ε exonuclease and polymerase domains (pol2-16) survive, but have severe growth defects, checkpoint activation, increased level of dNTP pools as well as significant increase in the mutation rates. Herein, we suggest new opportunities to distinguish the roles of Pol ε from those of two other eukaryotic B-family DNA polymerases, Pols δ and ζ.