Open AccessIdentification of Iron Transporters Expressed in the Magnetotactic Bacterium Magnetospirillum magnetotacticum
Author(s) -
Azuma Taoka,
Chihiro Umeyama,
Yoshihiro Fukumori
Publication year - 2008
Publication title -
current microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.578
H-Index - 90
eISSN - 1432-0991
pISSN - 0343-8651
DOI - 10.1007/s00284-008-9305-7
Subject(s) - magnetotactic bacteria , magnetosome , ferrous , ferric , biology , siderophore , biogenesis , transporter , biochemistry , magnetite , bacteria , transport protein , transmembrane protein , bacterial outer membrane , cytoplasm , membrane transport protein , membrane protein , microbiology and biotechnology , atp binding cassette transporter , chemistry , escherichia coli , membrane , gene , receptor , genetics , inorganic chemistry , paleontology , organic chemistry
The magnetotactic bacterium Magnetospirillum magnetotacticum MS-1 mineralizes the magnetite (Fe(3)O(4)) crystal and organizes a highly ordered intracellular structure, called the magnetosome. However, the iron transport system, which supports the biogenesis of magnetite, is not fully understood. In this study, we first identified the expressions of both the ferric and the ferrous iron transporter proteins in M. magnetotacticum. The cellular protein compositions of ferric and ferrous iron-rich cultures were examined using two-dimensional electrophoresis. According to the gel patterns, two outer-membrane ferric-siderophore receptor homologues were identified as proteins strongly induced in the ferrous iron-rich condition. Also, we identified for the first time that the ferrous iron transport protein, FeoB, is expressed in the M. magnetotacticum cytoplasmic membrane using immunoblotting.
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