Open AccessReduction in Rat Oocyte Fertilizability Mediated by S-(1, 2-dichlorovinyl)-l-cysteine: A Trichloroethylene Metabolite Produced by the Glutathione Conjugation Pathway
Author(s) -
Katherine Lily Wu,
Trish Berger
Publication year - 2008
Publication title -
bulletin of environmental contamination and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.543
H-Index - 70
eISSN - 1432-0800
pISSN - 0007-4861
DOI - 10.1007/s00128-008-9509-3
Subject(s) - glutathione , metabolite , oocyte , trichloroethylene , toxicant , in vivo , chemistry , toxicity , pharmacology , biochemistry , biology , microbiology and biotechnology , environmental chemistry , genetics , enzyme , embryo , organic chemistry
Trichloroethylene (TCE), a commonly used industrial degreasing solvent and environmental toxicant, reduces rat oocyte fertilizability by an incompletely understood mechanism. Previous evidence implicated cytochrome P450 dependent oxidation of TCE. The current study investigated a second pathway, glutathione conjugation using S-(1,2-dichlorovinyl)-L-cysteine (DCVC), a mutagenic and cytotoxic TCE-metabolite. In vitro exposure of oocytes and in vivo exposure of females to DCVC significantly reduced oocyte fertilizability (63% vs. 26%; p < 0.005 and 60% vs. 36%; p < 0.005, respectively). Reduced fertilizability of oocytes following in vivo TCE exposure may be mediated partially by the glutathione conjugation pathway.