Evaluation of the fidelity of immunolabelling obtained with clone 5D8/1, a monoclonal antibody directed against the enteroviral capsid protein, VP1, in human pancreas | Zendy

Sarah J. Richardson | Zendy; Pia Leete | Zendy; Shalinee Dhayal | Zendy; Mark A. Russell | Zendy; Maarit Oikarinen | Zendy; Jutta E. Laiho | Zendy; Emma Svedin | Zendy; Katharina Lind | Zendy; Therese Rosenling | Zendy; Nora M. Chapman | Zendy; Adrian J. Bone | Zendy; Alan K. Foulis | Zendy; Gun Frisk | Zendy; Malin FlodströmTullberg | Zendy; Didier Hober | Zendy; Heikki Hyöty | Zendy; Noel G. Morgan | Zendy

Open Access

Evaluation of the fidelity of immunolabelling obtained with clone 5D8/1, a monoclonal antibody directed against the enteroviral capsid protein, VP1, in human pancreas

Author(s) -

Sarah J. Richardson,

Pia Leete,

Shalinee Dhayal,

Mark A. Russell,

Maarit Oikarinen,

Jutta E. Laiho,

Emma Svedin,

Katharina Lind,

Therese Rosenling,

Nora M. Chapman,

Adrian J. Bone,

Alan K. Foulis,

Gun Frisk,

Malin FlodströmTullberg,

Didier Hober,

Heikki Hyöty,

Noel G. Morgan

Publication year - 2013

Publication title -

diabetologia

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.464

H-Index - 227

eISSN - 1432-0428

pISSN - 0012-186X

DOI - 10.1007/s00125-013-3094-7

Subject(s) - biology , clone (java method) , microbiology and biotechnology , virology , monoclonal antibody , pancreas , blot , antibody , immunology , gene , genetics , biochemistry

Enteroviral infection has been implicated in the development of islet autoimmunity in type 1 diabetes and enteroviral antigen expression has been detected by immunohistochemistry in the pancreatic beta cells of patients with recent-onset type 1 diabetes. However, the immunohistochemical evidence relies heavily on the use of a monoclonal antibody, clone 5D8/1, raised against an enteroviral capsid protein, VP1. Recent data suggest that the clone 5D8/1 may also recognise non-viral antigens; in particular, a component of the mitochondrial ATP synthase (ATP5B) and an isoform of creatine kinase (CKB). Therefore, we evaluated the fidelity of immunolabelling by clone 5D8/1 in the islets of patients with type 1 diabetes.

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