Open AccessInsights from Drosophila on mitochondrial complex I
Author(s) -
Shauna-Kay Rhooms,
Anjaneyulu Murari,
Naga Sri Goparaju,
Maximino Vilanueva,
Edward Owusu-Ansah
Publication year - 2019
Publication title -
cellular and molecular life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.928
H-Index - 223
eISSN - 1420-9071
pISSN - 1420-682X
DOI - 10.1007/s00018-019-03293-0
Subject(s) - intermembrane space , mitochondrion , oxidative phosphorylation , bioenergetics , mitochondrial matrix , nad+ kinase , citric acid cycle , biology , biochemistry , inner mitochondrial membrane , chemiosmosis , electron transport chain , oxidoreductase , atp–adp translocase , mitochondrial intermembrane space , microbiology and biotechnology , neurodegeneration , oxidative stress , atp synthase , inner membrane , enzyme , disease , gene , bacterial outer membrane , cytosol , medicine , escherichia coli
NADH:ubiquinone oxidoreductase, more commonly referred to as mitochondrial complex I (CI), is the largest discrete enzyme of the oxidative phosphorylation system (OXPHOS). It is localized to the mitochondrial inner membrane. CI oxidizes NADH generated from the tricarboxylic acid cycle to NAD + , in a series of redox reactions that culminates in the reduction of ubiquinone, and the transport of protons from the matrix across the inner membrane to the intermembrane space. The resulting proton-motive force is consumed by ATP synthase to generate ATP, or harnessed to transport ions, metabolites and proteins into the mitochondrion. CI is also a major source of reactive oxygen species. Accordingly, impaired CI function has been associated with a host of chronic metabolic and degenerative disorders such as diabetes, cardiomyopathy, Parkinson's disease (PD) and Leigh syndrome. Studies on Drosophila have contributed to our understanding of the multiple roles of CI in bioenergetics and organismal physiology. Here, we explore and discuss some of the studies on Drosophila that have informed our understanding of this complex and conclude with some of the open questions about CI that can be resolved by studies on Drosophila.