Premium
Retention behavior of triglycerides on reverse phase columns using pseudophase liquid chromatography
Author(s) -
Singleton J. A.,
Pattee H. E.
Publication year - 1985
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf03028743
Subject(s) - chemistry , elution , chromatography , pulmonary surfactant , phase (matter) , adsorption , sodium dodecyl sulfate , micelle , cationic polymerization , aqueous solution , emulsion , micellar liquid chromatography , organic chemistry , biochemistry
The use of micellar mobile phases in decreasing the elution time of peanut triglycerides on 10µ reverse phase columns is demonstrated. Both cationic and anionic surfactants were used with “end‐capped” and “non‐end‐capped” reverse phase columns. Surfactants added to both nonaqueous and aqueous organic mobile phases reduced the elution time of all triglyceride peaks when compared to a mobile phase without a surfactant modifier. Sodium dodecyl sulfate (SDS) as the modifier in the mobile phase was the most effective in reducing elution time for triglycerides when an “end‐capped” column was used as the stationary medium. Increasing concentrations of surfactant in the mobile phase resulted in corresponding decreases in elution times. A unique feature of micellar phases is that any increase in concentration only alters the concentration of the micelles in the mobile phase. The objective of this study was to investigate the retention behavior of large, non‐ionic solute molecules and to elucidate the adsorption mechanism on reverse phase columns.