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High performance size exclusion chromatography of monomer, dimer and trimer mixtures
Author(s) -
Christopoulou C. N.,
Perkins E. G.
Publication year - 1989
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf03022759
Subject(s) - trimer , chromatography , dimer , monomer , divinylbenzene , size exclusion chromatography , chemistry , gel permeation chromatography , styrene , copolymer , toluene , refractometry , organic chemistry , materials science , polymer , refractive index , enzyme , optoelectronics
A high performance size exclusion Chromatographic method was developed for the separation of monomer, dimer and trimer fatty acids in thermally oxidized or used fats and oils. The system was composed of two styrene/divinylbenzene copolymer columns with toluene as the mobile phase and refractometry as the mode of detection. The internal standardization method was used for quantitation with monostearin as the internal standard. Quantitation of monomer, dimer and trimer content in various samples correlated well with results obtained by GLC analysis and gravimetically by size exclusion chromatography.