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Fatty Acid Profile Analysis of Membrane Phospholipids of Isolated Soybean Lipid Bodies
Author(s) -
Simpson T. D.
Publication year - 1989
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf03022751
Subject(s) - phosphatidylethanolamine , phosphatidic acid , phospholipid , phosphatidylcholine , phosphatidylinositol , chemistry , biochemistry , polyunsaturated fatty acid , phosphodiester bond , fatty acid , hydrolysis , enzyme , membrane , rna , kinase , gene
Phospholipids in isolated soybean lipid bodies at 30‡C underwent degradation by acyl ester and phosphodiester hydrolysis and by phosphatidyl transfer. Under conditions that minimized oxidation, preferential loss of phosphatidylethanolamine and polyunsaturated fatty acids occurred, but acyl ester compositions of all phospholipids reflected enrichment of saturated and/or monounsaturated acids. Such fatty acid changes in phosphatidylinositol, which was degraded less than phosphatidylcholine or phosphatidylethanolamine, and in phosphatidic acid, which accumulated, also suggest that transesterification occurred extensively in lipid bodies. The specific temporal changes in phospholipid and acyl chain composition suggest that several enzymes remained active in isolated lipid bodies.