Premium
Studies on applications of lypolytic enzymes in detergency II. Evaluation of adaptability of various kinds of lipases in practical laundry conditions
Author(s) -
Tatara Takako,
Fujii Tomiko,
Kawase Tokuzo,
Minagawa Motoi
Publication year - 1985
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02935716
Subject(s) - lipase , hydrolysis , triolein , mucor , chemistry , laundry , tributyrin , yeast , chromatography , organic chemistry , enzyme , biochemistry , food science , penicillium , waste management , engineering
Kinetic parameter of hydrolysis, degree of hydrolysis, pH and temperature characteristics and positional specificity of hydrolysis of seven kinds of lipases from various microorganisms and a pancreatic lipase were examined for triolein or olive oil emulsion as a substrate and the adaptability of lipases for laundry systems was evaluated on the basis of these properties. As a result, it was found that lipases from yeast of Candida cylindracea (Lipase MY® and OF®) were excellent in kinetic parameter and degree of hydrolysis and temperature characteristics and had no positional specificity, and that lipases from mold of Mucor (Lipase M‐AP® and SP®) were excellent in pH and temperature characteristics and had positional specificity. On the viewpoint of the present laundry practice under alkaline conditions and the tendency toward low temperature washing, lipases from Candida and Mucor seem to be more suitable for laundry systems in comparison with lipases from other microorganisms and a pancreatic lipase. The effect of positional specificity in hydrolysis of lipase will be discussed on the basis of examination on the removal of triglyceride and its hydrolysates by surfactant solution in a subsequent paper.