Expanded-bed adsorption utilizing ion-exchange resin to purify extracellular β-galactosidase | Zendy

José Antônio Marques Pereira | Zendy; Paulo de Tarso Vieira e Rosa | Zendy; Gláucia María Pastore | Zendy; César Costapinto Santana | Zendy

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Expanded-bed adsorption utilizing ion-exchange resin to purify extracellular β-galactosidase

Author(s) -

José Antônio Marques Pereira,

Paulo de Tarso Vieira e Rosa,

Gláucia María Pastore,

César Costapinto Santana

Publication year - 1998

Publication title -

applied biochemistry and biotechnology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.558

H-Index - 111

eISSN - 1559-0291

pISSN - 0273-2289

DOI - 10.1007/bf02920188

Subject(s) - adsorption , chromatography , chemistry , ion exchange resin , centrifugation , elution , ion exchange , filtration (mathematics) , fermentation , expanded bed adsorption , extracellular , ion , biochemistry , organic chemistry , statistics , mathematics

The application of expanded-bed ion-exchange resins allows the elimination of intermediary particulate separation steps like filtration or centrifugation prior to adsorption steps in enzyme-purification processes from crude fermentation broths. This work is concerned with the experimental evaluation data of a process related to the adsorption of an extracellular p-galactosidase from the fungi Scopulariopsis. The protein recovery in the ion-exchange resin Accell Plus QMA was accomplished using a continuous-monitoring method. The direct adsorption step was followed by a elution step with concentrated NaCl solutions aiming to improve the enzyme-specific activity. Experimental data for fixed and expanded bed were compared.

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