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Gas chromatographic determination of free fatty acids in vegetable oils by a modified esterification procedure
Author(s) -
Chapman G. W.
Publication year - 1979
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02914273
Subject(s) - transesterification , chemistry , methanol , chromatography , titration , fatty acid , vegetable oil , gas chromatography , organic chemistry
Free fatty acids in small vegetable oil samples were determined by gas liquid chromatography after a modified BCl 3 or BF 3 /methanol esterification procedure with methylurea. This compound was found to sufficiently reduce triacylglyceride transesterification during free fatty acid esterification with BC1 3 or BF 3 methanol. Good results were obtained using this new procedure on neutral lipid oils containing known amounts of free fatty acids. Good agreement between the new method and the A.O.C.S. titration method was also demonstrated on commercial and laboratory‐extracted crude oils. Because very small oil samples (200 mg) can be analyzed, the new method could be applicable for oilseed physiological studies.