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Gossypol analysis in cottonseed oil by HPLC
Author(s) -
Chamkasem N.
Publication year - 1988
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02912562
Subject(s) - gossypol , chromatography , chemistry , high performance liquid chromatography , cottonseed , cottonseed oil , solvent , elution , hexane , absorbance , extraction (chemistry) , organic chemistry , biochemistry , food science
Abstract Gossypol in cottonseed oil was selectively separated by the extraction of cottonseed oil with a hexane and N,N dimethyl formamide:water (2∶1, v/v) solvent mixture. After filtration, the extract was injected into the HPLC with the elution time less than 15 min. The spectrophotometric method showed 2 to 5 times higher values of gossypol content in different types of glanded cottonseed oil than did the HPLC method. This is probably due to the gossypol derivatives and coloring interferences reacting with p‐anisidine to develop color and increasing the absorbance reading, whereas gossypol was separated and detected in the HPLC method.