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Direct analysis of trivernolin by high‐performance liquid chromatography
Author(s) -
Plattner R. D.,
Wade K.,
Kleiman R.
Publication year - 1978
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02911895
Subject(s) - chromatography , acetone , acetonitrile , calibration curve , chloroform , chemistry , high performance liquid chromatography , analytical chemistry (journal) , organic chemistry , detection limit
A rapid liquid chromatographic method for the quantitation of trivernolin in epoxy seed oils has been developed. A weighed sample of the oil is dissolved in chloroform (∼100–300 mg/ml), and a 1–20 µ l portion of the oil is chromatographed on a 2 ft×1/4 in. OD C 18 µ ‐Bondapak column with acetonitrile‐acetone (2:1, v/v). The area of the trivernolin peak from the differential refractometer recorder tracing is converted to µ g trivernolin by use of a calibration curve of peak area vs. trivernolin weight.