Direct analysis of trivernolin by high‐performance liquid chromatography | Zendy

Plattner R. D. | Zendy; Wade K. | Zendy; Kleiman R. | Zendy

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Direct analysis of trivernolin by high‐performance liquid chromatography

Author(s) -

Plattner R. D.,

Wade K.,

Kleiman R.

Publication year - 1978

Publication title -

journal of the american oil chemists' society

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.512

H-Index - 117

eISSN - 1558-9331

pISSN - 0003-021X

DOI - 10.1007/bf02911895

Subject(s) - chromatography , acetone , acetonitrile , calibration curve , chloroform , chemistry , high performance liquid chromatography , analytical chemistry (journal) , organic chemistry , detection limit

A rapid liquid chromatographic method for the quantitation of trivernolin in epoxy seed oils has been developed. A weighed sample of the oil is dissolved in chloroform (∼100–300 mg/ml), and a 1–20 µ l portion of the oil is chromatographed on a 2 ft×1/4 in. OD C 18 µ ‐Bondapak column with acetonitrile‐acetone (2:1, v/v). The area of the trivernolin peak from the differential refractometer recorder tracing is converted to µ g trivernolin by use of a calibration curve of peak area vs. trivernolin weight.

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