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Semiautomatic analysis of serum triglycerides and cholesteryl esters by infrared absorption
Author(s) -
Freeman N. K.,
Lampo E.,
Windsor A. A.
Publication year - 1967
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02908361
Subject(s) - chemistry , chromatography , fraction (chemistry) , extraction (chemistry) , analytical chemistry (journal) , adsorption , absorption (acoustics) , infrared spectrophotometry , materials science , organic chemistry , composite material
Abstract An instrument has been developed for the semiautomatic analysis of mixtures of triglycerides and cholesteryl esters. The method is based on high‐resolution infrared spectrophotometry, and has previously been shown to be applicable to the determination of these components in the nonionic fraction of human serum lipids. A simple nonrecording grating spectrophotometer has been suitably modified to carry out this analysis; and appropriate computing circuitry has been coupled with it for performing the two‐component calculation. The supplementary electronics consist of operational amplifiers, a logarithmic conversion circuit, a digital voltmeter, and a printer. Automatic operation is accomplished by a control mechanism, which programs the measurements, the steps in the calculations, and print‐out of the results. Sample preparation consists of an extraction of lipids from serum in such a way as to exelude phospholipids. This may be done in a single step, although a two‐step procedure—total lipid extraction followed by adsorption separation of the phospholipids—appears to be more reliable. Measurements are made on a solution of the neutral lipid fraction in carbon tetrachloride.