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Estimation of crude fiber in dehulled soybeans
Author(s) -
Anderson R. E.,
Holt K. E.
Publication year - 1967
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02901255
Subject(s) - fiber , microscope , sample (material) , cellulose , acetic acid , materials science , extraction (chemistry) , chromatography , pulp and paper industry , chemistry , composite material , optics , engineering , organic chemistry , biochemistry , physics
The determination of crude fiber in soybeans is a lengthy and time‐consuming procedure which requires extraction, digestion, and incineration and is not suitable as a routine processing‐control tool. A short control procedure has been developed, employing photomicroscopy, which is based on the characteristics of soybean hull cellulose to rotate plane‐polarized light. The sample is mounted on a microscope slide, treated with trichloro‐acetic acid, and placed in a polarizing projection microscope; the image is compared with a series of standard photomicrographs. The amount of hulls present in the sample is measured quantitatively, and an estimation is made on the crude fiber. The crude fiber estimation on de‐hulled soybeans can be made in 15 min compared with 8 to 12 hr by using the official crude fiber method.