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Further observations on the 2‐thiobarbituric acid method for the measurement of oxidative rancidity
Author(s) -
Yu T. C.,
Sinnhuber R. O.
Publication year - 1964
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02898128
Subject(s) - thiobarbituric acid , oxidizing agent , chemistry , reagent , decomposition , pigment , oxidative phosphorylation , chromatography , tbars , oxidative stress , organic chemistry , food science , biochemistry , lipid peroxidation
Abstract The validity of the 2‐thiobarbituric acid (TBA) procedure for the measurement of oxidative rancidity by the determination of malonaldehyde (MA) as the red pigment with an absorption max at 532 m Ɓ has been questioned [J. Am. Oil Chem. Soc. 39 , 34 (1962)]. Side reactions were reported to occur yielding degradation of TBA to products which absorb at the same wave length as the TBA‐MA complex and at 450 m Ɓ . Results reported in the present paper stress the importance of reagent purification. Little or no decomposition of TBA to produce interfering colors was found after heating with acids, oxidizing agents or hydroperoxides.