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Determination of TBA number by high performance liquid chromatography
Author(s) -
Kakuda Y.,
Stanley D. W.,
Voort F. R.
Publication year - 1981
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02887320
Subject(s) - high performance liquid chromatography , chromatography , chemistry , absorbance , correlation coefficient , aqueous solution , distillation , standard curve , repeatability , analytical chemistry (journal) , organic chemistry , mathematics , statistics
A high performance liquid chromatographic (HPLC) method for the quantitation of malonaldehyde in aqueous distillates was developed. Compared with the standard TBA test, the HPLC method was faster, and less affected by side feactions. A total of 5 min was necessary to assay each distillate and only malonaldehyde was detected. The standard curves were reproducible and standards were stable for up to 6 days. The HPLC method could detect malonaldehyde levels ranging from 1×10 −11 to 4×10 −11 mol/10 µ L and either peak height or peak area could be used to quantitate the malonaldehyde concentration. The coefficient of determination between absorbance values determined by the TBA test and peak heights determined by HPLC was 0.946. Twenty‐one freeze‐dried chicken samples with TBA numbers ranging from 3.93 to 16.6 were used for this correlation.