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Simultaneous detection of aflatoxin b 1 and ochratoxin a in olive oil
Author(s) -
Letutour B.,
TantaouiElaraki A.,
Ihlal L.
Publication year - 1983
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02787439
Subject(s) - aflatoxin , ochratoxin a , mycotoxin , ochratoxin , chemistry , chromatography , ochratoxins , olive oil , contamination , clean up , extraction (chemistry) , chloroform , thin layer chromatography , food science , biology , ecology
A screening method has been developed for simultaneous determination of aflatoxin B 1 and ochratoxin A in olive oil. The technique includes extraction of both mycotoxins with aqueous methanol, clean‐up using lead acetate, partitioning in chloroform, and thin layer chromatography. The detection limits achieved are, respectively: 4 μg aflatoxin B 1 and 40 μg ochratoxin A/kg. 60 samples of crude farm olive oil were analyzed using this method: three of them contained traces of ochratoxin A while none was contaminated with aflatoxin B 1 .