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Comparative evaluation of solvent extraction methods for the determination of neutral and polar lipids in beef
Author(s) -
Sahasrabudhe M. R.,
Smallbone B. W.
Publication year - 1983
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02787431
Subject(s) - chemistry , chromatography , petroleum ether , extraction (chemistry) , chloroform , silicic acid , solvent , methanol , ether , fatty acid , biochemistry , organic chemistry
Abstract Samples of lean (< 5% fat), medium (13–15%) and high‐fat (> 20%) ground beef were extracted for total lipid by 4 methods of wet extraction employing chloroform/methanol (CM), n‐hexane/iso‐propanol (HIP) and ethyl alcohol/ethyl ether (AE), and by 3 methods of soxhlet extraction of freeze‐dried material by petroleum ether (PE) or eithyl ether (EE), CM and methylene chloride/ methanol (MM). The purified lipid was fractionated into neutral and polar lipid fractions by silicic acid chromatography and the fractions were analyzed for fatty acid distribution by gas liquid chroma‐tography (GLC). The soxhlet procedure employing either PE or EE extracted less than 75% of total lipid, 89% of triglycérides and 15% of polar lipids from lean beef as compared to other methods, and as the fat content increased from 3 to 20%, extracted amounts of polar lipid which increased to 40% of that extracted by other methods. The fatty acid distribution of the fractionated triglycerides and polar lipids was generally within experimental error for each frac‐tion, irrespective of the method of extraction. The percentages of 16:0 and 18:1 were significantly less in polar lipids than in trigly‐cerides. In addition to significantly higher percentage of 18:2, the polar lipids contained up to 20% of long‐chain fatty acids not detected in triglycerides. The soxhlet procedures with CM or MM were as effective as wet extraction procedures in extracting neutral and polar lipids.