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Separation of fatty acid methyl esters on the basis of the number of double bonds using alumina plates
Author(s) -
Lowry R. R.,
Tinsley I. J.
Publication year - 1983
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02787429
Subject(s) - double bond , isomerization , ozonolysis , chemistry , fatty acid , hexane , molecule , chromatography , solvent , thin layer chromatography , methyl oleate , silica gel , organic chemistry , catalysis
The separation of fatty acid methyl esters (FAME) on the basis of the number of double bonds present in the molecule was accom‐plished using thin layer chromatography (TLC) on alumina. Hexane was used as the solvent in a continuous development mode using modified tanks. The separations were verified by gas liquid chroma‐tography (GLC) and retention of structural integrity was confirmed by infrared analysis for trans isomerization and by ozonolysis‐GLC for double bond position.