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Improved separation of natural oil triglycerides by liquid chromatography using columns packed with 3‐μm particles
Author(s) -
Dong M. W.,
Dicesare J. L.
Publication year - 1983
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02787428
Subject(s) - chromatography , packed bed , resolution (logic) , detector , chemistry , analytical chemistry (journal) , alkyl , materials science , optics , organic chemistry , physics , artificial intelligence , computer science
Very high‐resolution separations of triglycerides in various natural oils have been demonstrated by liquid chromatography using short columns packed with 3‐μm alkyl bonded‐phase particles. Analysis times range from 8 to 16 min without prior sample clean‐up. The primary detector used was a refractive index detector having low dispersive characteristics. Both the high efficiency of the columns and the selectivity of the 3‐μ packing material contribute to the separation of several critical pairs of triglycerides. A substantial reduction in analysis time was also achieved. An ultraviolet detector operated at 220 nm was used to illustrate an alternative detection approach.