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Variables in the technicon‐N‐24 method for the determination of serum cholesterol
Author(s) -
Roland Dorothy,
Cooper Gerald R.,
Witter Robert F.
Publication year - 1966
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02682562
Subject(s) - autoanalyzer , reagent , chromatography , chemistry , isopropyl alcohol , cholesterol , analytical chemistry (journal) , biochemistry , organic chemistry
A modification of the Technicon‐N‐24 method for serum cholesterol is described. It is possible to obtain precise results which agree on the average with those obtained with the reference Abell‐Kendall method. The level of cholesterol found by the AutoAnalyzer method depends primarily on the ratio of the rates of flow of the lipid extract to that of the premixed color reagent and the time of heating of the color reaction at 95C. In this modification, the flow rate of isopropyl alcohol extract of sample or standard is kept at 0.9 ml/min and the ratio of flow of extract to that of premixed color reagent is maintained between 1蝘10 and 1蝘12 while the time of heating of the color reaction is maintained between 95 and 120 sec. Other conditions necessary for precise results are discussed. The precision obtained on the average in several experiments ranged from 2.2 to 2.7 mg% as standard deviation between duplicates.