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Quantification of carbonyl compounds in oxidized fats as trichlorophenylhydrazones
Author(s) -
White Pamela J.,
Hammond Earl G.
Publication year - 1983
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02680352
Subject(s) - chemistry , chromatography , decomposition , elution , ether , gas chromatography , chloride , cyclohexane , organic chemistry
A rapid determination of the carbonyl compounds in oxidized fats and oils was devised based on formation of trichlorophenylhydrazones (TCPH). Fat in cyclohexane/ether (99:1) was passed through a Florisil column to remove interfering hydrocarbons, and the fat and carbonyl compounds were eluted with ether. The eluate was reacted with trichlorophenylhydrazine with Florisil as a catalyst, and the TCPH were isolated from the fat by chromatography on a Florisil column. The TCPH were separated and quantified by gas chromatography on a 10‐m SE 30 capillary column. The method was applied to soybean oils stored under various conditions. The method also was applied to linoleic acid hydroperoxide to determine the extent to which decomposition of peroxides gave rise to carbonyl artifacts during the procedure. Although artifacts were minimal, they were reduced even further by passing the fat through a column of Celite impregnated with stannous chloride to reduce hydroperoxides before the carbonyl analysis.