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The Determination of Fatty Acid Primary Amides by Capillary Gas Chromatography
Author(s) -
Brengartner Dennis A.
Publication year - 1986
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02679597
Subject(s) - derivatization , chromatography , gas chromatography , chemistry , capillary gas chromatography , fatty acid , reproducibility , extraction (chemistry) , primary (astronomy) , high performance liquid chromatography , organic chemistry , astronomy , physics
A method has been developed for the determination of 12 primary amides of long‐chain fatty acids by capillary column gas chromatography. The method uses no derivatization or sample preparation other than extraction of the sample. A variety of commercial amidecontaining materials have been analyzed successfully. The amides, along with other soluble materials, are first separated from the host using refluxing 2‐propanol containing an internal standard. The fatty acid amides are then identified and measured by gas chromatography over a programmed temperature range of 200 to 260 C. The chromatograms obtained show sharp peaks, unique retention times and acceptable reproducibility for quantitation. Cis‐ trans isomers of several of the fatty acid amides were tested and found to be resolved under the conditions employed.