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Pons scaled‐down clean‐up column adapted for use in solvent‐saving modification of the CB method for aflatoxin
Author(s) -
Lee L. S.,
Catalano E. A.
Publication year - 1981
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02679297
Subject(s) - aflatoxin , chromatography , solvent , chemistry , column (typography) , volume (thermodynamics) , clean up , mathematics , extraction (chemistry) , organic chemistry , food science , physics , geometry , connection (principal bundle) , quantum mechanics
The solvent‐saving procedure devised by Pons using a small Chromatographie tube (Bio‐Rad Laboratories glass Econo‐Column, 10 mm id X 300 mm long) has been adapted and extended for use in modifications of the Official AOAC procedure for quantitative determination of aflatoxins in corn, peanuts, soybeans, coconut and pistachios. Thirty mL of each of 3 solvents for column washes was used instead of the 150 mL specified by the Official CB Method. The analytical aliquot was also reduced 80%, but sample size and extracting solvent volume were not changed, so that there was no loss in sensitivity. Toxins ranging from 3 to over 1,000 μ g/g of sample were quantitated after clean‐up using both procedures with no statistically significant difference between results.