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Thin‐layer chromatographic determination of antioxidants by the stable free radical ॅ, ॅ′‐diphenyl‐ॆ‐picrylhydrazyl
Author(s) -
Glavind J.,
Holmer G.
Publication year - 1967
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02679243
Subject(s) - saponification , dpph , chemistry , chromatography , antioxidant , tocopherol , thin layer chromatography , organic chemistry , thin layer , vitamin e , layer (electronics)
The reaction between the stable free radical ॅ,ॅ′‐diphenyl‐ॆ‐picrylhydrazyl (DPPH) and antioxidants can be used for the visualization of antioxidants in thin‐layer chromatography. Amounts of tocopherols down to 0.5 ॖg can be detected. The identification of tocopherols in oils and other natural products and of synthetic antioxidants, added to lard, are described. Saponification is not required. Quantitative analyses of े‐tocopherol give satisfactory results.