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Behavior of diglycerides and conjugated fatty acid triglycerides in reverse‐phase chromatography
Author(s) -
Chang M. K.,
Conkerton E. J.,
Chapital D.,
Wan P. J.
Publication year - 1994
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02675915
Subject(s) - glyceride , chemistry , chromatography , conjugated system , fatty acid , elution , phase (matter) , column chromatography , high performance liquid chromatography , gas chromatography , organic chemistry , polymer
The behavior of conjugated fatty acid triglycerides and diglycerides on reverse‐phase chromatography was studied. Trieleostearin is a geometric isomer of trilinolenin. The conjugated double bond arrangement in trieleostearin enhances its hydrophobic interaction with the stationary phase and causes it to be eluted later than trilinolenin. In separation of “critical pairs” of tri‐ and diglycerides, diglycerides elute later than triglycerides due to the longer fatty acid constituent. Position isomers of 1,2‐ and 1,3‐diglycerides can be separated by reverse‐phase high‐performance liquid chromatography.