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Optimization of parameters for the analysis of triglyceride by reverse phase HPLC using a UV detector at 210 nm
Author(s) -
Singleton J. A.,
Pattee H. W.
Publication year - 1984
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02672130
Subject(s) - chemistry , high performance liquid chromatography , phase (matter) , chromatography , methanol , solvent , chloroform , elution , resolution (logic) , matrix (chemical analysis) , reversed phase chromatography , analytical chemistry (journal) , organic chemistry , artificial intelligence , computer science
Factors affecting the quantitative analysis of triglycerides from natural oils were investigated on reverse‐phase HPLC columns. The organic solvent used to dissolve the triglycerides greatly determined the extent to which the solute molecules interacted with the mobile phase and hydrocarbonaceous ligand. Chloroform‐dissolved triglycerides resulted in better resolution and detection of the solute matrix of all the organic solvents used. Also, ethanol was superior to methanol as the polar organic modifier in the mobile‐phase composition. Ballistic temperature programming significantly reduced analysis time and column temperature, when raised high enough, can reverse the elution of some solutes, Possible mechanisms of triglyceride solute retention on reverse‐phase columns are advanced in conjunction with the various parameters used in the investigation.