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Compositional analysis of natural wax ester mixtures by tandem mass spectrometry
Author(s) -
Spencer Gayland F.,
Plattner Ronald D.
Publication year - 1984
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02672053
Subject(s) - chemistry , wax , mass spectrometry , isobutane , protonation , tandem mass spectrometry , fragmentation (computing) , collision induced dissociation , chromatography , mass spectrum , dissociation (chemistry) , ion , fourier transform ion cyclotron resonance , analytical chemistry (journal) , organic chemistry , computer science , operating system , catalysis
Abstract Tandam mass spectrometry is particularly suited for the analysis of complex, natural wax ester mixtures R 1 ‐CO 2 ‐R 2 . Reduction of the mixture with deuterium provides species that are separble (through mass spectrometry) based on the number of original double bonds. Chemical ionization with isobutane produces high yields of protonated molecular ions and very little further fragmentation. These ions are separated by the first mass filter and then dissociated through collisions with argon. The positively charged dissociation products are almost exclusively the protonated acid ions (R 1 ‐CO 2 H 2 ) + that can then be separated by the second mass filter before detection and quantitation. The technique overcomes many of the obstacles previously faced during wax ester analysis. Results from this method are compared with those obtained by previous work, and the isomer composition of a new wax ester oil, orange roughy oil, is given. DIRECT SUPPORT A03O2445 00003