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Isolation of isomeric hydroperoxides from the peanut lipoxygenase‐ linoleic acid reaction
Author(s) -
Pattee Harold E.,
Singleton John A.
Publication year - 1977
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02670793
Subject(s) - linoleic acid , lipoxygenase , chemistry , mass spectrometry , organic chemistry , chromatography , enzyme , fatty acid
Hydroperoxides were isolated from the peanut lipoxygenase‐linoleic acid reaction mixture and were separated as their methyl esters by high performance liquid chromatography. Mass spectrometry and infra‐red analysis indicated the isolated hydroperoxides to be 13‐hydroperoxy‐ cis ‐9, trans‐ 11‐octadecadienoic acid; 13‐hydroperoxy‐ trans‐ 9, trans‐ 11‐octadeca‐dienoic acid; and 9‐hydroperoxy‐ trans ‐l0, trans‐ 12 ‐ octadecadienoic acid. The percentages of the hydro‐peroxides in the reaction mixture were 72.8%, 3.6%, and 23.6% under the conditions used. 1 Paper No. 4973 of the Journal Series of the North Carolina Agricultural Experiment Station, Raleigh, NC 27607.