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High pressure liquid chromatography for the analysis of soluble carbohydrates in defatted oilseed flours
Author(s) -
Cegla G. F.,
Bell K. R.
Publication year - 1977
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02670783
Subject(s) - stachyose , raffinose , chemistry , chromatography , sucrose , sugar , cottonseed , disaccharide , high performance liquid chromatography , food science , organic chemistry
The soluble carbohydrate content of four defatted oilseed flours (cottonseed, soybean, peanut, and sun‐flower) was determined by using high pressure liquid chromatography. The best method of separating the sugars from the other components of water extracted flour was found to be thin layer chromatography. Plates of Silica Gel H with a running solvent of iso‐propanol‐water‐ehtyl acetate (54:26:20) gave good isolation of the sugars. The μ BONDAPAK/ Carbohydrate column was used to analyze the sugars. An eluting solvent of acetonitrile and water (75:25 and 72:28) at a flow rate of 1.5 ml/min gave the best resolution of the carbohydrates. The major carbohy‐drates in the oilseed flours were glucose, sucrose, raffinose, and stachyose. Small amounts of trehalose and an unidentified disaccharide were found in the flour of sunflower.