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Determination of chlorophyll pigments in crude and degummed canola oils by HPLC and spectrophotometry
Author(s) -
Daun J. K.,
Thorsteinson C. T.
Publication year - 1989
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02670098
Subject(s) - canola , pheophytin , chlorophyll , chlorophyll a , chromatography , high performance liquid chromatography , chlorophyll b , chemistry , pigment , spectrophotometry , botany , food science , biology , photosynthesis , biochemistry , organic chemistry , photosystem ii
Chlorophyll pigments in crude and degummed canola oils were analyzed by spectrophotometry using a modified AOCS Method and by reversed phase HPLC. HPLC showed that crude canola oils contained very little chlorophyll a or b , these pigments having been converted to pheophytins and other pigments with similar spectral properties. The ratio of chlorophyll a∶b in the seed was found to be about 3∶1 while the ratio of pheophytin a∶b in the oil was about 9∶1. As the AOCS Method for determining oil chlorophyll was calibrated for pure chlorophyll, the use of this method on crude canola oil results in a significant error. Recalibration of the spectrophotometric procedure with pheophytin gave better agreement with the HPLC method.