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The determination of lactic acid in shortening containing lactylated glycerides, by liquid‐liquid partition chromatography
Author(s) -
Buswell R. J.
Publication year - 1964
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02670020
Subject(s) - saponification , chemistry , chromatography , lactic acid , silicic acid , chloroform , glyceride , titration , sulfuric acid , fatty acid , organic chemistry , bacteria , genetics , biology
Shortenings containing ca. 1% lactic acid added as lactylated glycerides are analyzed for total or water insoluble combined lactic acid (WICLA) by a liquid‐liquid partition Chromatographie pro‐cedure. The sample is saponified, acidified with H 2 SO 4 , dispersed on silicic acid, slurried with chloroform and transferred to the top of a chro‐matographic column composed of a 0.5 N sulfuric acid stationary phase supported on silicic acid. After elution of the fatty acids by chloroform, 15% m‐butanol removes the excess H 2 SO 4 fol‐lowed by a well resolved lactic acid fraction. Titration of the latter fraction with standard methanolic sodium hydroxide has shown the lactic acid recovery to be 94%. The WICLA determina‐tion requires 7 hr and has a standard deviation of ŷ0.020%.